다양한 소독방법이 PPDO-hybrid-PLGA nanofiber scaffold의 형태와 세포부착에 미치는 영향에 관한 연구
EFFECT OF DIFFERENT STERILIZATION METHODS ON THE SURFACE MORPHOLOGY OF PPDO-hybrid-PLGA NANOFIBER SCAFFOLD AND ATTACHMENTS OF PC12 CELL
이주현, 민현기, 정주영, 강나라,
소속 상세정보
이주현 ( Lee Ju-Hyon ) - 이화여자대학교 의학전문대학원 구강악안면외과
민현기 ( Min Hyun-Gi ) - 이화여자대학교 의학전문대학원 구강악안면외과
정주영 ( Jeong Ju-Young ) - 이화여자대학교 임상치의학대학원 임플란트학과
강나라 ( Kang Na-Ra ) - 이화여자대학교 임상치의학대학원 임플란트학과
KMID : 0355620080340060635
Abstract
Objectives: the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated.
Methods: Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at 65 ℃ , and Group Ⅲ: scaffold sterilized with Ethylene Oxide at 37 ℃. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at 65 ℃, and Group II: sterilized with Ethylene Oxide at 37 ℃.
Results: 1. The surface morphology was slightly changed in Group I, II and GroupⅢ, compared with control.
2. The attachment of PC12 cells in Group I, II was not higher than in control
Discussion: The attachment of PC12 cell is not influenced by different sterilization methods.
키워드
Sterilization;Nanofiber scaffold;Surface morphology;Attachment of cell
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